MBP-
301 MICROBIAL GENETICS AND MOLECULAR BIOLOGY
TOTAL HOURS: 48
CREDITS: 2
COURSE
OUTCOMES:
CO1:Understand
about the isolation of genetic elements.
Co2:
Know about various characteristical process and their micro process.
1.
Study of different
types of DNA and RNA using micrographs and model / schematic representations
2.
Study of
semi-conservative replication of DNA through micrographs / schematic representations
3.
Isolation of genomic
DNA from E. coli
5. Resolution
and visualization of DNA by Agarose Gel Electrophoresis.
6. Resolution
and visualization of proteins by Polyacrylamide Gel Electrophoresis
(SDS-PAGE).
7.Problems related to
DNA and RNA characteristics, Transcription and Translation.
SUGGESTED
READING
Crueger, W. and Crueger, A.
(2000). Biotechnology: A Text Book of Industrial Microbiology, PrenticeHall
of India Pvt. Ltd., New Delhi.
Freifelder, D.
(1990). Microbial Genetics. Narosa Publishing House, New Delhi.
Freifelder, D. (1997). Essentials
of Molecular Biology. Narosa Publishing House, New Delhi.
Glazer, A.N. and
Nikaido, H. (1995). Microbial Biotechnology – Fundamentals of Applied
Microbiology, W.H. Freeman and company, New York.
Glick, B.P. and
Pasternack, J. (1998). Molecular Biotechnology, ASM Press, Washington
D.C., USA.
Kannan, N. (2003). Hand
Book of Laboratory Culture Medias, Reagents, Stains and Buffers. Panima
Publishing Co., New Delhi.
Lewin, B. (2000). Genes
VIII. Oxford University Press, England
Maloy, S.R.,
Cronan, J.E. and Freifelder, D. (1994). Microbial Genetics, Jones and
Bartlett Publishers, London.
Nicholl, D.S.T.
(2004). An Introduction to Genetic Engineering. 2 nd Edition.
Cambridge University Press, London.
Old, R.W. and
Primrose, S.B. (1994) Principles of Gene Manipulation, Blackwell
Science
B.Sc MICROBIOLOGY (CBCS) SYLLABUS
SECOND YEAR – SEMESTER- IV
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MBT- 401 IMMUNOLOGY AND MEDICAL MICROBIOLOGY
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TOTAL HOURS: 48 CREDITS: 4
COURSE
OUTCOMES:
CO1:
Understand various cells and types immune proess that helps to keep immunity
in a organism
CO2:
Know about various types of bacterial fungal and viral diseases.
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UNIT-I No. of hours: 10
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Types
of immunity – innate and acquired; active and passive; humoral and
cell-mediated immunity.
Primary
and secondary organs of immune system – thymus, bursa fabricus, bone marrow,
spleen and lymph nodes.
Cells
of immune system.
Identiification
and function of B and T lymphocytes, null cells, monocytes, macrophages,
neutrophils, basophils and eosinophils.
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UNIT-II No. of hours: 10
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Antigens
– types, chemical nature, antigenic determinants, haptens.
Factors affecting antigenicity.
Antibodies
– basic structure, types, properties and functions of immunoglobulins.
Types
of antigen-antibody reactions - Agglutinations, Precipitation,
Neutralization, complement fixation,
blood groups.
Labeled antibody based techniques – ELISA,
RIA and Immunofluroscence. Polyclonal
and monoclonal antibodies –
production and applications.
Concept
of hypersensitivity and Autoimmunity.
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UNIT-III No.
of hours: 10
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Normal flora of human body.
Host pathogen interactions: infection,
invasion, pathogen, pathogenicity, virulence and opportunistic infection.
General account on nosocomial
infection.
General principles of diagnostic
microbiology- collection, transport and processing of clinical samples.
General methods of laboratory
diagnosis - cultural, biochemical, serological and molecular methods.
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UNIT-IV
No.
of hours: 8
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Antibacterial Agents- Penicillin, Streptomycin and Tetracycline.
Antifungal agents – Amphotericin B,
Griseofulvin Antiviral substances -
Amantadine and Acyclovir Tests for antimicrobial susceptibility. Brief
account on antibiotic resistance in bacteria - Methicillin-resistant Staphylococcus aureus (MRSA). Vaccines – Natural and recombinant. General account on microbial diseases – causal organism,
pathogenesis, epidemiology, diagnosis, prevention and control
Bacterial diseases –
Tuberculosis and TyphoidFungal
diseases – Candidiasis.Protozoal diseases – Malaria.Viral Diseases -
Hepatitis- A and AIDS.
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MBP-
401 IMMUNOLOGY AND MEDICAL MICROBIOLOGY
TOTAL
HOURS: 48
CREDITS:
2
COURSE
OUTCOMES:
CO1: Understand
various serological techniques
Co2: Know about
about various stages and sympotms of a disease.
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1.
Identification of human blood groups.
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2.
Separate serum from the blood sample (demonstration).
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3.
Estimation of blood haemoglobin.
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4.
Total Leukocyte Count of the given blood sample.
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5.
Differential Leukocyte Count of the given blood sample.
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6.
Immunodiffusion by Ouchterlony method.
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7.
Identify bacteria (E. coli, Pseudomonas, Staphylococcus,
Bacillus) using laboratory strains on the basis of cultural,
morphological and biochemical characteristics: IMViC, urease production and
catalase tests
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Isolation of
bacterial flora of skin by swab method.
|
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Antibacterial
sensitivity by Kirby-Bauer method
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8.
Study symptoms of the
diseases with the help of photographs: Anthrax,
Polio, Herpes, chicken pox, HPV warts, Dermatomycoses (ring worms)
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9.
Study of various
stages of malarial parasite in RBCs using permanent mounts.
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SUGGESTED READING
Abbas AK,
Lichtman AH, Pillai S. (2007). Cellular
and Molecular Immunology. 6th edition Saunders Publication, Philadelphia.
Ananthanarayan
R. and Paniker C.K.J. (2009) Textbook of
Microbiology. 8th
edition,
University Press Publication
Brooks
G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013)
Jawetz,
Melnick and Adelberg’s Medical
Microbiology. 26th edition. McGraw Hill
Publication
Delves
P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology.11th edition Wiley-Blackwell Scientific
Publication, Oxford.
Goering R., Dockrell H., Zuckerman M.
and Wakelin D. (2007) Mims’ Medical
Microbiology.
4th edition. Elsevier
B.Sc MICROBIOLOGY (CBCS) SYLLABUS
THIRD YEAR – SEMESTER- V
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MBT- 501 ENVIRONMENTAL &
AGRICULTURAL MICROBIOLOGY
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TOTAL
HOURS: 36 CREDITS: 3
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COURSE
OUTCOMES:
CO1:
Able to know about various environmental aspects in our surroundings.
CO2:Understand
the role of microorganisms in varius treatment process of water purification.
CO3:Knowleadgeble
about various terrestrial,Aquatic and extreme habitat of microorganisms.
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UNIT - I
No. of hours: 8
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Terrestrial
Environment: Soil profile and soil microflora
Aquatic
Environment: Microflora of fresh water and marine habitats
Atmosphere:
Aeromicroflora and dispersal of microbes
Extreme
Habitats: Extremophiles: Microbes thriving at high & low temperatures,
pH, high hydrostatic & osmotic pressures, salinity, & low nutrient
levels.
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UNIT – II
No. of hours: 8
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Role
of microorganisms in nutrient cycling (Carbon, nitrogen, phosphorus).
Treatment and safety of drinking
(potable) water, methods to detect potability of water samples: (a) standard
qualitative procedure: presumptive test/MPN test, confirmed and completed
tests for faecal coliforms (b) Membrane filter technique.Microbial
interactions – mutualism, commensalism, antagonism, competition, parasitism,
predation.
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UNIT
– III No. of hours: 6
|
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Outlines of Solid Waste management:
Sources and types of solid waste, Methods of solid waste disposal (composting
and sanitary landfill).
Liquid waste management: Composition and
strength of sewage (BOD and COD), Primary, secondary (oxidation ponds,
trickling filter, activated sludge process and septic tank) and tertiary
sewage treatment.
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UNIT
– IV No.
of hours: 14
|
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Plant
Growth Promoting Microorganisms - Mycorrhizae, Rhizobia, Azospirillum,
Azotobacter, Frankia, phosphate-solubilizers and Cyanobacteria.
Outlines
of biological nitrogen fixation (symbiotic, non-symbiotic).
Biofertilizers
- Rhizobium.
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Concept
of disease in plants. Symptoms of
plant diseases caused by fungi, bacteria, and viruses. Plant diseases - groundnut rust, Citrus
canker and tomato leaf curl.
Principles
of plant disease control.
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MBP- 501 ENVIRONMENTAL &
AGRICULTURAL MICROBIOLOGY
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TOTAL HOURS: 36
CREDITS: 2
COURSE OUTCOMES:
CO1: Understand the various flora of
microorganisms
CO2: Know about varioud Isolaation techniques of
Microorganisms.
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1. Analysis of soil – pH, Moisture content and
water holding capacity.
|
2. Isolation of microbes (bacteria and fungi)
from soil.
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3. Study of air flora by petriplate exposure
method.
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4.
Analysis of potable water: SPC, Presumptive, confirmed and completed
test, determination of coliform
count in water by MPN.
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5. Determination of Biological Oxygen Demand
(BOD) of waste water samples.
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6.
Isolation of Rhizobium from root nodules.
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7.
Staining and observation of
Vesicular Arbuscular Mycorrhizal (VAM) fungi.
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8. Observation of plant diseases of local importance -
Citrus canker, Tikka disease of
Groundnut, Bhendi yellow vein mosaic, Rusts, Smuts, Powdery mildews,
Tomato leaf
curl.
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SUGGESTED
READINGS
Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals &
Applications. 4th edition.
Benjamin/Cummings Science Publishing, USA
Barton LL & Northup DE
(2011). Microbial Ecology. 1st
edition, Wiley Blackwell, USA
Campbell RE. (1983). Microbial Ecology. Blackwell Scientific
Publication, Oxford, England.
Coyne MS.
(2001). Soil Microbiology: An
Exploratory Approach. Delmar Thomson Learning.
Lynch JM & Hobbie JE. (1988). Microorganisms in Action: Concepts &
Application in Microbial Ecology. Blackwell Scientific Publication, U.K.
Madigan MT, Martinko JM and Parker J.
(2014). Brock Biology of Microorganisms.
14th edition. Pearson/ Benjamin
Cummings
Maier RM, Pepper IL and Gerba CP.
(2009). Environmental Microbiology. 2nd
edition, Academic Press
Martin A. (1977). An Introduction to Soil Microbiology. 2nd
edition. John Wiley & Sons Inc. New York & London.
Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st
edition, Springer, New York.
Singh A, Kuhad, RC & Ward OP (2009).
Advances in Applied Bioremediation.
Volume 17, Springer-Verlag, Berlin Hedeilberg
B.Sc MICROBIOLOGY (CBCS) SYLLABUS
THIRD YEAR – SEMESTER- VII
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MBT- 601 FOOD
AND INDUSTRIAL MICROBIOLOGY
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TOTAL HOURS: 36 CREDITS: 3
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COURSE
OUTCOMES:
CO1:
Understand various food borne diseases occurred by microorganisms
CO2:
Know about various production process of food poducts.
CO3:
Acquire knowledge about various preservation processes
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UNIT- I No. of hours: 8
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Intrinsic and extrinsic parameters
that affect microbial growth in food
Microbial spoilage of food - fruits,
vegetables, milk, meat, egg, bread and canned foods
Food
intoxication (botulism).
Food-borne
diseases (salmonellosis) and their detection.
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UNIT – II No. of hours: 7
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Principles
of food preservation - Physical and chemical methods.
Fermented
Dairy foods – cheese and yogurt.
Microorganisms
as food – SCP, edible mushrooms (white button, oyster and paddy straw).
Probiotics and their benefits.
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UNIT – III No. of hours: 6
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Microorganisms
of industrial importance – yeasts, moulds, bacteria, actinomycetes.
Isolation
and Screening of industrially-important microorganisms.
Outlines
of strain improvement.
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UNIT – IV
No.
of hours: 15
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Types
of fermentation processes – solid state, liquid state, batch, fed-batch,
continuous.
Design
of fermenter.
Ingredients
of Fermentation media
Downstream
processing - filtration, centrifugation, cell disruption, solvent extraction.
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Microbial
production of Industrial products - Citric acid, Ethanol, amylases,
penicillin, glutamic acid and vitamin B12.
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MBP- 601 FOOD
AND INDUSTRIAL MICROBIOLOGY
TOTAL HOURS: 36
COURSE OUTCOMES:
CO1: Understand various
isolations of microorganisms in food products
CO2: Know about various microbial
fermentation processes CREDITS:
2
1. Isolation of bacteria and fungi
from spoiled bread/fruits/vegetables
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2.
Preparation of Yogurt/Dahi
3.
Determination of the microbiological quality of milk sample by MBRT
4.
Isolation of antagonistic microorganisms by crowded plate technique
5.
Design of Fermenter
6.
Microbial fermentation for the production and estimation of ethanol from
Grapes.
7.
Microbial fermentation for the production and estimation of citric acid.
SUGGESTED
READING
Adams MR and Moss MO.
(1995). Food Microbiology. 4th
edition, New Age International (P) Limited Publishers, New Delhi, India.
Banwart JM. (1987). Basic Food Microbiology. 1st edition.
CBS Publishers and Distributors, Delhi, India.
Casida
LE. (1991). Industrial Microbiology.
1st edition. Wiley Eastern Limited.
Crueger W and Crueger
A. (2000). Biotechnology: A textbook
of Industrial Microbiology. 2nd Edition. Panima Publishing Company, New Delhi
Frazier WC and
Westhoff DC. (1992). Food Microbiology.
3rd edition. Tata McGraw-Hill Publishing Company Ltd, New Delhi, India.
Jay JM, Loessner MJ
and Golden DA. (2005). Modern Food
Microbiology. 7th edition, CBS Publishers and Distributors, Delhi,
India
Patel AH. (1996). Industrial Microbiology .1st Edition.
MacMillan India Limited Publishing Company Ltd. New Delhi, India
Stanbury PF, Whitaker
A and Hall SJ. (2006). Principles of
Fermentation Technology. 2nd edition, Elsevier Science Ltd.
Tortora GJ, Funke BR,
and Case CL. (2008). Microbiology: An introduction. 9th Edition.
Pearson Education
Willey JM, Sherwood LM
AND Woolverton CJ (2013), Prescott, Harley and Klein’s Microbiology. 9th Edition. McGraw Hill Higher education
MBP-
801 MICROBIAL QUALITY CONTROL IN FOOD AND PHARMACEUTICAL INDUSTRIES
TOTAL HOURS:
36 CREDITS: 2
COURSE OUTCOMES:
Co1: Understand
various sterility test to be done for the identification of a microorganism
CO2: Know
about various sterility tests for instruments.
1.
Microbiological laboratory safety- General rules & Regulations.
2.
Sterility tests for Instruments – Autoclave & Hot Air Oven
3. Disinfection of selected instruments
& Equipments
4.
Sterility of Air and its relationship to Laboratory & Hospital sepsis.
5.
Sterility testing of Microbiological media
6.
Sterility testing of Pharmaceutical products –Antibiotics, Vaccines &
fluids
7. Standard qualitative analysis of water.
8.
Quantitative analysis of water – Membrane filter method
9.
Analysis of food samples for Mycotoxins
SUGGESTED READING
Baird
RM, Hodges NA and Denyer SP (2005) Handbook of Microbiological Quality
control in Pharmaceutical and Medical Devices, Taylor and Francis Inc.
Garg
N, Garg KL and Mukerji KG (2010) Laboratory Manual of Food Microbiology I K
International Publishing House Pvt. Ltd.
Harrigan
WF (1998) Laboratory Methods in Food Microbiology, 3rd ed. Academic Press
Jay
JM, Loessner MJ, Golden DA (2005) Modern Food Microbiology, 7th edition.
Springer
Laboratory Exercises
in Microbiology, George.A.Wistreich & Max.D.Lechtman, 3 rd Ed, Glencoe
press, London.
Manual of diagnostic
microbiology, Dr.B.J.Wadher & Dr.G.L.Bhoosreddy, Firs.Ed., Himalaya
publishing house, Nagpur.
Microbiology - A
laboratory manual, Cappuccino & Sherman , 6 th Ed, Pearson Education
Pharmaceutical
Microbiology – Purohit
B.Sc
MICROBIOLOGY (CBCS) SYLLABUS
THIRD
YEAR – SEMISTER-VIIIB
MBT-
803 BIOFERTILIZERS AND BIOPESTICIDES
TOTAL
HOURS: 36 CREDITS: 3
COURSE
OUTCOMES:
CO1:
Understand about various applications of biofertilizers and pesticides
CO2:
Know about varius important plant microbes
UNIT – I No
of Hours: 10
General
account of the microbes used as biofertilizers for various crop plants and
their advantages over chemical fertilizers.
Symbiotic
N2 fixers: Rhizobium -
Isolation, characteristics, types, inoculum production and field application,
legume/pulses plants
Frankia from non-legumes and characterization.
Cyanobacteria from Azolla, characterization, mass multiplication, Role in rice
cultivation, Crop response, field application.
UNIT – II No of Hours: 6
Free
living Azospirillum, Azotobacter - isolation,
characteristics, mass inoculum production and field application.
UNIT – III No
of Hours: 6
Phosphate
solubilizing microbes - Isolation, characterization, mass inoculum
production, field application
UNIT – IV No
of Hours: 14
Importance
of mycorrizal inoculum, types of mycorrhizae and associated plants, Mass
inoculum production of VAM, field applications of Ectomycorrhizae and VAM.
General account of microbes used as
bioinsecticides and their advantages over synthetic pesticides. Bacillus thuringiensis - production,
Field applications.
Viruses
– NPV cultivation and field applications.
MBP-
803 BIOFERTILIZERS AND BIOPESTICIDES
TOTAL HOURS: 36 CREDITS: 2
COUIRSE OUTCOMES:
CO1:
Understand isolation process of pvarious plant microbes
CO2;
Know about various staining methods of plant microbes
1. Isolation
of Rhizobium from root nodules.
3.
Isolation of phosphate solubilizers from soil
4. Staining and observation of VAM
3. A
visit to biofertilizer production
unit.
SUGGESTED READINGS
Agarwal SK (2005) Advanced Environmental Biotechnology,
APH publication.
Kannaiyan,
S. (2003). Bioetchnology of
Biofertilizers, CHIPS, Texas.
Mahendra K. Rai (2005). Hand book of Microbial biofertilizers,
The Haworth Press, Inc. New York.
Reddy, S.M. et. al. (2002). Bioinoculants for sustainable agriculture
and forestry, Scientific Publishers.
Saleem F and Shakoori AR (2012)
Development of Bioinsecticide, Lap
Lambert Academic Publishing GmbH KG
Subba
Rao N.S (1995) Soil microorganisms and
plant growth Oxford and IBH publishing co. Pvt. Ltd. NewDelhi.
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